Coding

Part:BBa_K1975001:Design

Designed by: Joachim Steen Larsen   Group: iGEM16_UNIK_Copenhagen   (2016-06-28)


PCT - Propionate CoA-transferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
    Illegal XhoI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To ensure as high expression as possible the gene sequence has been codon optimized for Bacillus subtilis. This was done using the codon optimization tool from IDT.


Source

This gene is originally from a genomic sequence from Pseudomonas sp. 61-3.

References